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Nothing is new up until room 60, where the player finds the "express" note on the table. However, instead of releasing Specimen 2, reading the note creates a door to the Express Tunnel, which will send the player to Room 512. It should be noted that specimens can spawn in the short walk from Room 50 to 60.
At Room 556, the player will stumble upon a giant, stone-like cubic structure covered in strange symbols. Inside it (Room 557), there are several doors the player can open. Going through the right door leads to a room with a record player that can be played, which causes W.A.M to appear. In the room to the left, the player can find a key (not in the HD Renovation, however, as it's not necessary) to open the middle door. Going through the middle door leads the player to Room 558 (557 for the HD Renovation), where they have to use the axe to break the boards nailed to the door.Once the player reaches Room 559, (557 for the HD Renovation) Specimen 8 appears in the front hallway and begins to chase them. The rooms after this will revert back to normal.